Coating buffer(10 mM Bicarbonate buffer, pH 9.0; 10 mM PBS, pH 8.5)
Blocking reagent(2% BSA)
Sample Diluents(for Serum, plasma, Antibodies, lysates, extracts, etc. – 10 mM PBS, pH 7.4)
Wash Buffer(PBS-Tween 20 – 10 mM PB with 0.9% saline and 0.1% Tween 20)
Stop Solutions(2N Sulfuric Acid, 2 N Phosphoric Acid)
TMB Substrate- 1x, 5x, 10x(Single Component for HRP)
pNPP(for Alkaline Phosphatase)
ABTS Substrate(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid for HRP)
Secondary Antibodies conjugated with HRP or Alkaline Phosphatase
Streptavidin-HRP Conjugate